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I'Screen STREPTO quantifies streptomycin and dihydrostreptomycin in milk, honey and royal jelly

06 August 2012

A new kit for aminoglycosides residues screening is available after I’Screen Neomycin and I’Screen Gentamicin.
I’Screen STREPTO: a new fast test kit for quantitative detection of streptomycin and dihydrostreptomycin. Honey, royal jelly and milk are already validated.

Streptomycin and dihydrostreptomycin are aminoglycoside antibiotic drugs used in apiculture for the treatment of bacterial brood diseases, e.g. American foulbrood. This practice is forbidden in Europe. Moreover, streptomycin is increasingly used to treat important fruit-tree diseases such as fireblight and Pseudomonas blossom blast. Contamination of the blossom with high concentrations of antimicrobials implies the risk of a carry-over of residues into the honey and the royal jelly. Residues of these antibiotics may lead to a potential hazard to the exposed consumer in terms of toxicity, allergic reaction and the development of bacterial resistance. Tecna R&D Laboratories developed and validated a competitive enzyme immunoassay for the quantitative detection of streptomycin and dihydrostreptomycin in honey and royal jelly. The assay can detect both aminoglycosides with > 100% cross-reactivity.

The assay implementation takes one hour. The honey preparation is based on liquid buffer extraction without any clean up step and specific reagents requirements. The assay can detect 5 ppb of streptomycin in honey (CCβ validation was performed according to the European Decision 657/2002). Concerning royal jelly, a specific acid extraction was developed to guarantee the same sensitivity at 5 ppb of streptomycin.

The same CCβ of 5 ppb was finally obtained for milk, thus achieving 40 times higher sensitivity than European Regulation 37/2010 and Codex Alimentarius 32nd session requirements (200 ppb MRL). A specific zero standard was featured for milk analysis with I’Screen STREPTO: it is already available on the market with cod. AB654. Thanks to the introduction of this buffer, no sample manipulation is required for milk, with the only exception of a defatting step.

Thanks to its easiness-to-use, I’Screen STREPTO (Cod. AB650) was awarded on April 2012 with the Innovation Prize 3T/3L by AREA Science Park Consortium. The assay is available on the market in a 96 determinations format. For any further clarification and for requiring the Performance Data Sheet, contact the technical assistance at export@tecnalab.com.