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I'Screen SULFA
cod. SM390

Description Enzyme immunoassay for the detection of sulphonamides.

The kit has been designed and developed with the aim to detect all the principal sulphonamides used in zootechnics.
Codes
96 determinations format SM390
Assay principle Microplate competitive enzyme immunoassay.
Analysable samples Muscle, Egg, Milk, Honey, Feed.
Detection limit
Muscle, Egg (METHOD I) and honey (METHOD III, high sensitivity): 0.5 ppb
Milk (METHOD I) 0.25 ppb
Muscle, Egg, Milk, Honey (METHOD II, solvent-free): 5 ppb
Feed: 66.6 ppb
Assay time 75 minutes
Sample preparation
Muscle, Egg, milk (METHOD I, high sensitivity)
Homogenization, (skimming for milk), solvent extraction, centrifugation, evaporation.
Muscle, egg, honey (METHOD II, solvent free) Homogenization, (acid hydrolysis for honey) buffer extraction, centrifugation, filtration.
Milk (METHOD II) Centrifugation and dilution.
Honey (METHOD III) Acidic hydrolysis, purification on SPE column, elution, evaporation.
Feed: Grinding, Solvent extraction, dilution.
Specificity
Sulfamerazine > 100%
Sulfamonomethoxine > 100%
Sulfadiazine > 100%
Sulfachloropyridazine > 100%
Sulfamethoxydiazine (sulfamethoxine, sulfameter) > 100%
Sulfamethoxypyridazine (sulfapyridazine) > 100%
Sulfadimethoxine > 100%
Sulfaquinoxaline > 100%
Sulfathiazole > 100%
Sulfamethizole > 100%
Sulfamethazine 100%
Sulfamethoxazole 90%
Sulfisoxazole 50%
Sulfapyridine 36%
Sulfadoxine 24%
Sulfacetamide 2.5%
Sulfaphenazole < 2%
Sulfabenzamide < 2%
Sulfaguanidine < 0.1%
Sulfanilamide < 0.1%
Last update September 2016
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