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B ZERO DON v2
cod. MD102-MD104

Description ELISA test kit for the quantitative detection of deoxynivalenol (vomitoxin).
Codes
48 determinations format MD102
96 determinations format MD104
Assay principle Microplate competitive enzyme immunoassay without standard curve.

Sample quantification through interpolation of inhibition value on virtual curve (master-curve) provided for each batch of kit.
Analysable samples Cereals, Feed, DDGS ("distillers").
Detection limit
Wheat, Maize, Feed and DDGS: 0.04 ppm
Durum wheat: 0.12 ppm
Wheat bran and Middlings: 0.24 ppm
Assay time 20 minutes
Sample preparation Cereals (Maize, Wheat), Feed, DDGS: Grinding, methanol - water extraction, filtration.

Durum wheat: Grinding, water extraction, centrifugation, dilution.

Wheat bran and Middlings: Water extraction, activated charcoal clean-up, filtration, dilution.
Specificity
Deoxynivalenol 100%
3-acetyl-deoxynivalenol > 100%
3-glucosyl-deoxynivalenol 64%
15-acetyl-deoxynivalenol 2%
Nivalenol < 4%
Last update November 2016
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